Lumiprobe AF488 NHS ester (替代Alexa Fluor® 488)
  • Lumiprobe AF488 NHS ester (替代Alexa Fluor® 488)

AF488 NHS ester


AF488是一种明亮且耐光的染料,是Alexa Fluor® 488的替代产品。由于其高亲水性,因此可用于标记敏感蛋白和抗体。

修饰基团: NHS ester
包装规格: 1 mg



AF488 is a bright and photostable dye, equivalent of Alexa Fluor® 488. Due to its high hydrophilicity, this is a dye of choice for the labeling of sensitive proteins and antibodies. The dye is useful for many demanding applications, including microscopy.

From the chemical standpoint, AF488 is a sulfonated rhodamine dye Rhodamine 110 (R110). Like other rhodamines, it is available as 5- and 6-isomers, which have almost identical photophysical properties. The isomers need to be separated though - otherwise, use of mixed isomer dye can lead to doubled peaks during HPLC or electrophoresis separations of the labeled products. This product is an isomerically pure 5-AF488.

This NHS ester is an amine reactive dye, it can label amine groups in proteins, peptides, amino-modified oligos, and other target molecules.

AF488是一种明亮且耐光的染料,是Alexa Fluor® 488的替代产品。由于其高亲水性,因此可用于标记敏感蛋白和抗体。该染料可用于许多苛刻的应用,如显微镜。

从化学方面讲,AF488是磺化罗丹明染料若丹明110(R110)。像其他罗丹明一样,它存在5-和6-异构体,它们具有几乎相同的光物理性质。2种异构体需要分离,否则,使用混合的异构体染料可能会在HPLC或电泳分离标记产物期间导致峰倍增。本产品AF488 NHS ester是纯的异构体5-AF488。