Lumiprobe dsGreen for Real-Time PCR 实时定量PCR用染料
  • Lumiprobe dsGreen for Real-Time PCR 实时定量PCR用染料
  • qPCR curves with dsGreen

dsGreen for Real-Time PCR, 100×

¥546.00
含13%增值税(专票和普票价格相同)

dsGreen是SYBR®Green I的类似物,是一种非常敏感的dsDNA检测染料。对dsDNA的高灵敏度和高选择性,可以将dsGreen用作qPCR的通用dsDNA检测试剂。无需使用标记的探针来检测dsGreen的扩增。

包装规格
数量
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dsGreen, an analog of SYBR® Green I, is a very sensitive dsDNA detection dye. High sensitivity, and high selectivity for dsDNA allow to use dsGreen as a universal dsDNA detection reagent for qPCR. No need to use labeled probes to detect amplification with dsGreen – unlabeled primers are sufficient.

Unlike other preparations of dsGreen provided by Lumiprobe for gel staining purposes, this formulation is specially designed to be used in real-time PCR experiments. Specific features are:

  1. Concentration of the dye is optimized for qPCR and carefully adjusted for reproducible results from lot to lot.
  2. PCR tested preparation – quality guaranteed
  3. Low fluorescence background – high fluorescence intensity gain

Note: the image of fluorescent properties of dsGreen bound to dsDNA are taken from the following publication: Zipper, H.; Brunner, H.; Bernhagen, J.; Vitzthum, F. Investigations on DNA intercalation and surface binding by SYBR Green I, its structure determination and methodological implications. Nucleic Acids Res., 2004, 32, e103.

dsGreen是SYBR®Green I的类似物,是一种非常敏感的dsDNA检测染料。对dsDNA的高灵敏度和高选择性,可以将dsGreen用作qPCR的通用dsDNA检测试剂。无需使用标记的探针来检测dsGreen的扩增-未标记的引物就足够了。

与Lumiprobe为凝胶电泳染色用dsGreen染料不同,该制剂经过特殊设计,可用于实时PCR实验。具体功能包括:

  1. 染料的浓度已针对qPCR进行了优化,并经过精心调整以实现批次之间可重复的结果。
  2. 经过PCR测试的制备物-质量保证
  3. 低荧光背景–高荧光强度增益
11010

详细信息

保存温度
冷冻 (-20C)
最大激发波长
454nm
最大发射波长
524nm
ε, L⋅mol−1⋅cm−1
73000
量子产率
0.8

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